hrp0089rfc10.4 | Late Breaking | ESPE2018

Comparative Analysis between Immunoassay and Tandem Mass Spectrometry for Androgens before and after Human Recombinant Gonadotrophin in Children with Genital Ambiguity and 46,XY Karyotype

Oliveira Leticia , Guerra-Junior Gil , Longui Carlos , Guaragna-Filho Guilherme , Costa Jose Luiz , Lanaro Rafael , Silva David , Mello Maricilda , Maciel-Guerra Andrea , Morcillo Andre

Liquid chromatography associated with tandem mass spectrometry (LC-MS/MS) is currently considered the gold standard for steroid measurement. The aim of this study was to compare traditional immunoassay and LC-MS/MS methods for androgens measurement before and after human recombinant chorionic gonadotrophin (hrCG) stimulation in children with diagnosis of disorder of sex development (DSD) with 46,XY karyotype and past of normal testosterone secretion. We evaluated 19 patients, ...